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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which ends up in chromosome instability, and therefore could contribute to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however available.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide typically has the epitope regarded through the antibody.
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This information outlines further achievable optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody alone, you can see which staining is distinct.
Goal Info Cyclin E belongs into the remarkably conserved cyclin household, whose members are characterised by a remarkable periodicity in protein website abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation styles which contribute towards the temporal coordination of each and every mitotic occasion. Cyclin E varieties a complex with and features to be a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and it is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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